Part:BBa_K1361006
Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
This part contains the major subunit of curli fiber CsgA and its nucleator CsgBtrunc, in which CsgA is constitutive expression whereas CsgBtrunc is under the control of Pbad promotor. CsgBtrunc is different from native CsgB from the deletion of C-terminal amino acid from 133 to 155 that it cannot attach cell outer membrane. And CsgBtrunc itself is highly aggregative in the culture. This part is designed to be used for immediate generate of large amount of curli fiber after adding of the inducer and a stronger promoter for CsgA was chosen to parallel with BBa_K1361002. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 707
Illegal NheI site found at 730 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |